Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HNRNPK

Cell type

Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
HNRNPK IP with antisense control, replicate 1
cell line
HCT116
treatment
antisense control
chip antibody
HNRNPK (Proteintech, Catalog: 11426-1-AP, Lot: 00022887)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After sequential RNase A and proteinase K treatment, DNA fragments were purified by phenol extraction and ethanol precipitation. The libraries were performed by using ThruPLEX® DNA-seq kit (Rubicon Genomics) according to manufacturer's instructions and applied to illumina platform for 151 nt pair-end sequencing by ABlife. Inc (Wuhan, China).

Sequencing Platform

instrument_model
HiSeq X Ten

hg38

Number of total reads
27860097
Reads aligned (%)
76.5
Duplicates removed (%)
17.1
Number of peaks
649 (qval < 1E-05)

hg19

Number of total reads
27860097
Reads aligned (%)
75.6
Duplicates removed (%)
17.4
Number of peaks
296 (qval < 1E-05)

Base call quality data from DBCLS SRA