Antigen

Antigen Class

TFs and others

Antigen

HNRNPK

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

HNRNPK IP with antisense control, replicate 1

cell line

HCT116

treatment

antisense control

chip antibody

HNRNPK (Proteintech, Catalog: 11426-1-AP, Lot: 00022887)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After sequential RNase A and proteinase K treatment, DNA fragments were purified by phenol extraction and ethanol precipitation. The libraries were performed by using ThruPLEX® DNA-seq kit (Rubicon Genomics) according to manufacturer's instructions and applied to illumina platform for 151 nt pair-end sequencing by ABlife. Inc (Wuhan, China).

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

27860097

Reads aligned (%)

76.5

Duplicates removed (%)

17.1

Number of peaks

649 (qval < 1E-05)

hg19

Number of total reads

27860097

Reads aligned (%)

75.6

Duplicates removed (%)

17.4

Number of peaks

296 (qval < 1E-05)